COVID-19

Reagents and Master Mixes

Empirical Bioscience’s QuantiTASE, RTScript™ Transcriptase, and InhibiTaq qPCR product lines have been tested and meet the performance requirements for 2019-nCoV detection as outlined by the CDC when used in conjunction with a EUA approved Primer & Probe Kit. Empirical Bioscience is an FDA registered and ISO13485:2016 certified manufacturer of Molecular Biology Reagents.

For Research Use Only.

Those using Empirical Bioscience products are responsible for ensuring they are used in compliance with the appropriate regulatory requirements.

Empirical Bioscience recommends that users within the USA jurisdiction follow the nCoV2/COVID testing guidelines established by the FDA and CDC. Links for current versions of these FDA and CDC documents are listed below.

Please note, these links are for informational purposes only. While we will do our best to make sure that links to these documents are up-to-date, we cannot guarantee they are the most recent versions. Users are responsible for verifying they are using the latest relevant information.

QuantiTASE PLUS Universal RT-qPCR Kit

The QuantiTASE PLUS Kit from Empirical Bioscience is an RT-qPCR Universal Kit that contains the reagents and MasterMixes necessary for accurately and efficiently synthesizing and amplifying cDNA from RNA samples. The reagents have been optimized for qPCR or RT-qPCR applications for use with both intercalating dye and probe-based detection methods. The QuantiTASE PLUS Kit is a high-performance version of the Empirical Bioscience QuantiTASE RT-qPCR Universal Kit. The Kit contains Empirical RTScript™ (M-MLV) Reverse Transcriptase Enzyme Mix and 4X InhibiTaq Plus HotStart qPCR MasterMix and 100mM DTT solution.

QuantiTASE Two-Step Reagent Kit

QuantiTASE Two-Step Reagent RT-qPCR Kits contain all the supplemental components necessary to perform first-strand cDNA synthesis with high sensitivity and efficiency from any RNA sample using two-step RT-qPCR protocols.

This kit is designed to be used in conjunction with Empirical’s RTScript™ Transcriptase Enzymes or QuantiTASE Kits.

QuantiTASE Two-Step RT-qPCR Reagent Kit
(100 Reactions)
Empirical’s RTScript™ is sensitive, specific, and is capable of synthesizing highly structured and long cDNA fragments. Produced cDNA is appropriate for qPCR analysis.

4X InhibiTaq qPCR MasterMix

Empirical’s InhibiTaq MasterMix is a 4X concentrated qPCR MasterMix containing Empirical’s proprietary HotStart Taq DNA polymerase and buffer system which has been developed specifically to permit robust qPCR performance in the presence of substances found in a wide range of samples that normally inhibit PCR amplification of DNA.

4X InhibiTaq PLUS qPCR MasterMix

Empirical’s InhibiTaq PLUS MasterMix is a 4X concentrated qPCR MasterMix containing Empirical’s proprietary HotStart Taq DNA Polymerase and Buffer System which has been developed specifically to permit robust qPCR performance in the presence of substances found in a wide range of samples that normally inhibit PCR amplification of DNA.

2X qPCR Probe MasterMix

Empirical’s qPCR Probe MasterMix is a 2X ready-to-use MasterMix for quantitative real-time evaluation of DNA using fluorescent probe-based detection. Empirical’s qPCR Probe MasterMix contains FlashTaq HotStart DNA Polymerase; a chemically modified Taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95°C. Empirical’s qPCR Probe MasterMix includes dNTPs, MgCl₂, and optimized buffer for fast, efficient qPCR. Empirical’s qPCR Probe MasterMix has been optimized for use with hydrolysis based probes such as TaqMan but is also suitable with other probe-based detection systems.

FlashTaq™ HotStart 2X MasterMix

Empirical’s FlashTaq™ HotStart 2X MasterMix is a 2X ready-to-use MasterMix that contains FlashTaq HotStart DNA Polymerase; a chemically modified HotStart Taq DNA polymerase with an activation time of 2 minutes at 94°C, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 3mM MgCl₂. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)

rEVAlution 2X qPCR MasterMix

Empirical’s rEVAlution is a ready to use hot-start qPCR MasterMix with EvaGreen® Dye, FlashTaq HotStart DNA polymerase, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 5mM MgCl2. EvaGreen® Dye is a dsDNA-binding dye. It is cell membrane impermeable, non-cytotoxic, non-mutagenic, and safe for aquatic life. EvaGreen® possesses similar absorption and emission spectra as SYBR® Green I. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)

RTScript™ cDNA Synthesis Kit

Empirical’s RTScript™ cDNA Synthesis Kit is a modified version of M-MLV reverse transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical’s RT enzyme synthesizes complementary DNA from single-stranded RNA or DNA templates.

Empirical’s RTScript™ is sensitive, specific, and capable of synthesizing highly-structured and long cDNA fragments. Produced cDNA is appropriate for both PCR and qPCR analysis.

RTScript™ cDNA Synthesis Kit
(100 reactions)
$372.82
Empirical’s RTScript™ cDNA Synthesis Kit is a modified version of M-MLV reverse transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical’s RT enzyme synthesizes complementary DNA from single-stranded RNA or DNA templates.

RTScript™ cDNA Transcriptase Enzyme

Empirical’s RTScript™ cDNA Transcriptase is a modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical’s RT enzyme synthesizes complementary DNA from single-stranded RNA or DNA templates.

ActiTemp-50 RTScript™ Reverse Transcriptase

Empirical’s ActiTemp-50 RTScript™ Reverse Transcriptase is a modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical’s RT enzyme synthesizes complementary DNA from single-stranded RNA or DNA templates.

Samples are available. Contact us today to discuss your requirements. Call 877.479.9949 for more information.

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