GENETIC ENGINEERING & BIOTECHNOLOGY NEWS: PCR Purification Kit

Empirical Bioscience’s new PCR Purification Kit streamlines the DNA purification process and delivers high-yield recovery. Designed with efficiency in mind, the kit arrives with the essential components including, binding buffer, activation buffer, elution buffer, washing buffer, spin columns, and collection tubes. It allows for efficient DNA purification, providing maximum recovery of linear or circular DNA ranging in size from 100 bp to 10 kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, loading dyes, and other impurities. Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer, and is designed to work with DNA amounts of up to 20 µg, so no organic extractions or additional precipitations are necessary. This helps to guarantee high and stable recovery rates.

Continue reading at Genetic Engineering & Biotechnology News

GENOMEWEB: Empirical Bioscience 2X qPCR Probe Master Mix

Empirical Bioscience has released 2X qPCR Probe Master Mix for probe-based quantitative PCR applications. This new product is for real-time evaluation of DNA using fluorescent probe-based detection. It contains FlashTaq HotStart DNA polymerase, a chemically modified Taq DNA polymerase that remains inactive at room temperature but becomes activated after 2 minutes at 95° C. The master mix also contains dNTPs, MgCL2, and optimized buffer for fast, efficient qPCR. It has been optimized for use with hydrolysis-based probes such as TaqMan, but is also suitable with other probe-based detection systems. The mix is available in multiple versions for various applications including bulk and custom volumes for commercial and biotech.

Continue reading at GenomeWeb.

PRESS RELEASE: New 2x Ready-to-Use Probe Master Mix Announced by Empirical Bioscience

2017—Empirical Bioscience, a designer and manufacturer of reagents for life science applications, has announced 2X qPCR Probe Master Mix for probe-based quantitative PCR applications. This new product is for real-time evaluation of DNA using fluorescent probe-based detection. It contains FlashTaq HotStart DNA Polymerase, a chemically modified Taq DNA polymerase that remains inactive at room temperature. The enzyme becomes activated after 2 minutes at 95°C. The master mix also contains dNTPs, MgCL2 and optimized buffer for fast, efficient qPCR. It has been optimized for use with hydrolysis based probes such as TaqMan, but is also suitable with other probe-based detection systems. The mix is available in multiple versions for various applications including bulk and custom volumes for commercial and biotech.

According to Marian Willing, Lead Scientist at Empirical Bioscience, “The 2X qPCR Probe Master Mix is both robust and sensitive. You can amplify down to 10 copies of HgDNA. It is compatible with most primer probe systems. Its low foam formula is ideal for automation. And it has been optimized so only primer/probe and template DNA need to be added. As a result, we believe it will be a welcome tool for scientists conducting real-time evaluations.”

Download full Press Release

PRESS RELEASE: New High Fidelity Blunt-End PFU-50 Polymerase from Empirical Bioscience Offers Precise, Rapid Amplification

Empirical Bioscience announced today that they have introduced PFU-50, an ultra high fidelity Polymerase for PCR application. In addition to delivering a fidelity that is 50 times greater than Taq polymerases and a rate of accuracy that is 2-fold higher than standard Pfu polymerases, PFU-50 also offers improved processing times, shorter elongation times, and better ligation.
The genetically engineered enzyme invokes the polymerization of nucleotides into duplex DNA in 5’?3’ direction but does not possess 5’?3’ exonuclease replacement activity. Instead, it offers a 3’?5’ exonuclease proofreading capability, significantly increasing the fidelity of DNA compared to Taq polymerase. This extensive exonuclease activity leaves the PCR fragments generated by PFU-50 High Fidelity Polymerase blunt, and without overhangs, for more effective linking of DNA. These characteristics lend themselves well to applications like cloning and next generation sequencing where accuracy, simple ligation, and speed are key.

“PFU-50 is ideal for applications that demand accurate replication of DNA,” said Marian Willing, Lead Scientist at Empirical Bioscience. “As well as those featuring long or difficult amplifications,” she added.

PFU-50 can be purchased on Empirical Bioscience’s Web site in units of 100 to 5,000. Free samples are also available. Empirical also offers this product, and all other products in bulk quantities

Download full Press Release

LABORATORY EQUIPMENT: Gel Extraction Kit Streamlines DNA Purification

Empirical Bioscience’s Agarose Gel Extraction Kit offers a simplified DNA purification process and robust results. Specifically designed to enhance efficiency, the kit arrives with the necessary components including extraction, activation, washing and elution buffers, as well as spin columns and collection tubes. The kit allows for efficient DNA purification from agarose gels and provides maximum recovery of DNA from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide and other impurities.

Continue reading at Laboratory Equipment.

GENOME WEB: Empirical Bioscience Plasmid Mini-Prep Kit

Empirical Bioscience recently introduced its Plasmid Mini-Prep Kit. The kit requires only 1 to 3 mL of bacterial culture, and isolates for extraction high-quality plasma or cosmid DNA up to 10 kb in length with yields of up to 20 µg per preparation. The kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches 7.5, the optimal pH level for DNA binding. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.

Continue reading at Genome Web.

BIOSPACE: Empirical Bioscience’s Plasmid Mini-Prep Kit Delivers Maximum Yields Using Minimal Culture

Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation.

In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.

Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in micro-centrifuges or on vacuum manifolds.

Continue reading at BioSpace.

GENETIC ENGINEERING & BIOTECHNOLOGY: New Products – Agarose Gel Extraction Kit

The new Agarose Gel Extraction Kit offers a simplified DNA purification process designed to enhance efficiency. The kit is supplied with the necessary components to streamline extraction workflows including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. The new kit allows for efficient DNA purification from agarose gels. The kit provides maximum recovery of DNA from 100 bp to 10 kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer so no organic extractions or additional precipitations are necessary—helping to insure high and consistent recovery rates.

Continue reading at GEN

BIO PORTFOLIO: Empirical Bioscience’s New Agarose Gel Extraction Kit Streamlines and Maximizes DNA Purification

Empirical Bioscience announced today that they have introduced a new Agarose Gel Extraction Kit that offers a simplified DNA purification process and robust results.

Specifically designed to enhance efficiency, the kit arrives with the necessary components including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes.

Continue reading at Bio Portfolio.

LAB BULLETIN: Empirical Bioscience’s New Agarose Gel Extraction Kit Streamlines and Maximizes DNA Purification

Empirical Bioscience announced today that they have introduced a new Agarose Gel Extraction Kit that offers a simplified DNA purification process and robust results.

Specifically designed to enhance efficiency, the kit arrives with the necessary components including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. Empirical Bioscience’s Agarose Gel Extraction Kit allows for efficient DNA purification from agarose gels. The kit provides maximum recovery of DNA from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities.

Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer so no organic extractions or additional precipitations are necessary. This helps to guarantee high and consistent recovery rates.

Continue reading at Lab Bulletin.