Empirical Bioscience T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).
Activity assay: 100ng of HindIII digested Lambda DNA is incubated in the presence of T4 ligase (600U, 120U) in 1X T4 Reaction Buffer for 30 min at 23°C. Ligation products are analyzed on a 1% agarose gel.
- High purity enzyme >99%
- No detectable endo or exo nuclease activity
- Manufactured under ISO 13485 quality controls
- Suitable for use as a raw material in IVD Applications
Source of Protein:
- A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C.