Empirical’s 10X GC Enhancer is a novel PCR cosolvent that enhances amplification of templates with up to 80% GC content. The 10X GC Enhancer is an excellent choice for multiplex reactions as it will overcome GC-rich targets without inhibiting other amplicons.
A GC-rich gene, ApoE, was amplified in singleplex with and without the 10X GC Enhancer. Using the 10X GC Enhancer, the fragment was correctly amplified without the multiple bands and primer dimers found when amplified without an enhancer.
Empirical’s 10X GC Enhancer allows GC-rich genes such as ApoE to be amplified. Multiplex reactions were run in triplicate with and without the 10X GC Enhancer. The ApoE was successfully amplified with the 10X GC Enhancer, but not without.
- High GC Content
- Enhances amplification of templates >70% GC.
- Multiplex Reactions
- Enhances GC-rich performance without inhibiting other amplicons.