PRESS RELEASE: Empirical Bioscience Plasmid Mini-Prep Kit Delivers Maximum Yields Using Minimal Culture

Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation.

In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.

Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in micro-centrifuges or on vacuum manifolds.

“The ability to use less bacterial culture to purify a sequence away from the genome and to refine the mixture for optimal DNA or plasmid isolation are significant advantages,” said Des O’Farrell, President of Empirical Bioscience. “Our kit has been shown to produce more high-quality plasmid than competitor brands that use more culture,” he added.

Empirical Bioscience produces high-grade PCR reagents and enzymes in its ISO 13485 certified facility in Grand Rapids, MI, USA and is a Registered Small Business Company.
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PRESS RELEASE: PCR Kit from Empirical Bioscience Simplifies and Optimizes DNA Purification

Empirical Bioscience announced today that they are now offering a PCR Purification Kit that streamlines the DNA purification process and delivers high-yield recovery.

Designed with efficiency in mind, the kit arrives with the essential components including, binding buffer, activation buffer, elution buffer, washing buffer, spin columns, and collection tubes. Empirical Bioscience’ PCR Purification Kit allows for efficient DNA purification. The kit provides maximum recovery of linear or circular DNA ranging in size from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, loading dyes, and other impurities.

Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer, and is designed to work with DNA amounts of up to 20 µg, so no organic extractions or additional precipitations are necessary. This helps to guarantee high and stable recovery rates.

“Our customer’s needs are our highest priority, and typically two of their main concerns are yield and efficiency,” said Des O’Farrell, President of Empirical Bioscience. “That’s why we kept ease-of-use and optimal recovery top-of-mind when we created this kit,”

Empirical Bioscience produces high-grade PCR reagents and enzymes in its ISO 13485 certified facility in Grand Rapids, MI, USA and is a Registered Small Business Company.

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GENOME WEB: Empirical Bioscience Agarose Gel Extraction Kit

Empirical Bioscience has launched the Agarose Gel Extraction kit. The kit includes an extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. It allows efficient DNA purification from agarose gels and provides maximum recovery of DNA from 100bp to 10kb. It also removes primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities, the company said.

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BIOSPACE: Empirical Bioscience’s New Agarose Gel Extraction Kit Streamlines And Maximizes DNA Purification

Empirical Bioscience announced today that they have introduced a new Agarose Gel Extraction Kit that offers a simplified DNA purification process and robust results.

Specifically designed to enhance efficiency, the kit arrives with the necessary components including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. Empirical Bioscience’s Agarose Gel Extraction Kit allows for efficient DNA purification from agarose gels. The kit provides maximum recovery of DNA from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities.

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PRESS RELEASE: Empirical Bioscience’s New Agarose Gel Extraction Kit Streamlines and Maximizes DNA Purification

Empirical Bioscience announced today that they have introduced a new Agarose Gel Extraction Kit that offers a simplified DNA purification process and robust results.

Specifically designed to enhance efficiency, the kit arrives with the necessary components including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. Empirical Bioscience’s Agarose Gel Extraction Kit allows for efficient DNA purification from agarose gels. The kit provides maximum recovery of DNA from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities.

Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer so no organic extractions or additional precipitations are necessary. This helps to guarantee high and consistent recovery rates.

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COMING SOON: New PFU-50 High Fidelity Polymerase

PFU-50 High Fidelity Polymerase

PFU-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered PFU DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. PFU-50 Polymerase-generated PCR fragments are blunt.

COMING SOON: 3 New Exciting Kits from Empirical Bioscience

PCR Purification Kits

Empirical Bioscience PCR Purification Kit is designed for the clean-up of PCR reactions by removal of primer dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify linear or circular DNA in the size range from 100 bp to 10 kb and is optimized for working with DNA amounts of up to 20 µg. The preparation requires no organic extractions or precipitation and guarantees high and stable recovery rates.

Plasmid Mini-Prep Kits

Empirical Bioscience Plasmid Mini-Prep Kit is designed for isolation of high-purity plasmid or cosmid DNA from bacterial cells for subsequent amplification, sequencing, restriction digests or transformations. The 2-step alkaline lysis procedure and binding column based preparation provide a fast, easy and efficient way of DNA isolation without shearing or significant loss of product. It allows elution in a small volume of low-salt buffer. Time-consuming phenol-chloroform extraction or alcohol precipitation are not required. The Lysis Buffer contains an integrated pH indicator to easily control the optimal pH value for DNA binding. Efficient DNA binding (for Column loading) requires a pH of 7.5 that is indicated by a color change to bright yellow. The kit can either be used in micro-centrifuges or on vacuum manifolds. It enables the extraction of plasmid DNA up to 10 kb length and yields up to 20 ?g DNA per preparation. The eluted high-quality plasmid DNA is ready to use for a variety of down-stream applications. For subsequent in vitro translation we recommend adding RNase Inhibitor or the application of an additional spin-column or phenol-chloroform based purification step. This avoids any risk of carry-over contamination with RNase due to the previous neutralization step.

Agarose Gel Extraction Kits

Empirical Bioscience Agarose Gel Extraction Kit is designed to extract high-yield DNA from agarose gels with simultaneous removal of primer dimers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify DNA in the size range from 100 bp to 10 kb. It requires no organic extractions or precipitation and guarantees high and stable recovery rates.

LABORATORY EQUIPMENT: Mixes Offer Enhanced Stability, Reduced Foaming

Empirical Bioscience’s line of Master Mix products have been optimized for enhanced activity, stability and reduced foaming. The improved products include MeanGreen, Multiplex, FlashTaq HotStart and rEVAlution qPCR 2X. Typically used in applications such as screening, cloning, or quantitative PCR, the low foam aspect of the product line allows for easier and more efficient use without the loss of material that can occur from bubble formation during the mixing/dispensing process. It also reduces the amount of material that can be lost from the adherence to plastic surfaces.

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GEN – GENETIC ENGINEERING & BIOTECHNOLOGY: cDNA Synthesis Kit

The RTScript™ is a cDNA Synthesis Kit that allows users to produce robust cDNA yields in as little as 30 minutes. This kit is a modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. These characteristics render it sensitive, specific, and capable of synthesizing highly structured and long cDNA fragments. The RTscript cDNA Synthesis Kit has also been optimized to offer high reproducibility and efficiency for a broad range of primers and templates. The kit contains all the reagents necessary for first strand cDNA synthesis including RNase-free water, ultrapure dNTPs, oligo (dT) primers, random hexamers, control RNA, and RNase inhibitor.

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PRESS RELEASE: Empirical Bioscience Elevates Detergent Free Master Mix Line with Enhanced Stability, Activity, and Low-Foam Qualities for PCR

Empirical Bioscience announced today that they have optimized their line of Master Mix products including, their MeanGreen, Multiplex, FlashTaq HotStart and rEVAlution qPCR 2X Master Mixes for enhanced activity, stability and reduced foaming.

Typically used in applications such as screening, cloning, or quantitative PCR, the low foam aspect of this product line allows for easier and more efficient use without the loss of material that can occur from bubble formation during the mixing/dispensing process. It
also reduces the amount of material that can be lost from the adherence to plastic surfaces.

What’s more, the low foam characteristic makes the Master Mixes easier to use and improves overall accuracy. The robust and highly-active nature of the Master Mix products also contributes to their overall ease of use. Formulated and manufactured without the use of detergents, the 2X Master Mix, 2X Taq Mean Green Master Mix, and Taq 5X Multiplex Master Mix may be stored and used at room temperature (15° to 25°C) for up to 90 days and for extended refrigeration storage at 4°C for up to 180 days. The 2X FlashTaq HotStart Master Mix, 2X Flash Taq HotStart Mean Green Master Mix, and 2X rEVAlution qPCR Master Mix products can be refrigerator stored at 4°C for 30 days. This not only reduces the degradation issues associated with freezing and thawing, but it also offers significant time-saving advantages and ease of use. This is especially beneficial in high throughput and automated applications where large volumes of Master Mix are needed for direct
use with their testing equipment.

Finally, the refined Master Mixes also offer improved efficiency and sensitivity. In fact, they can be used in reaction sizes as small as 10uL and they are capable of producing amplifications from as little as 60 picograms of DNA.

“Our ongoing effort to fine-tune our products and processes is evidence of our commitment to continue serving the laboratory users and biotech industry at the highest level,” said Des O’Farrell, President of Empirical Bioscience.

Empirical Bioscience produces high-grade PCR reagents and enzymes in its ISO 13485 certified facility in Grand Rapids, MI, USA and is a Registered Small Business Company.

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