Empirical’s qPCR Probe Master Mix is a 2x ready-to-use master mix for quantitative real time evaluation of DNA using fluorescent probe based detection. Empirical’s qPCR Probe Master Mix contains FlashTaq HotStart DNA Polymerase; a chemically modified Taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95°C. Empirical’s qPCR Probe Master Mix includes dNTPs, MgCl2, and optimized buffer for fast, efficient qPCR. Empirical’s qPCR Probe Master Mix has been optimized for use with hydrolysis based probes such as TaqMan, but is also suitable with other probe based detection systems.