KITS: Plasmid Mini-Prep
Empirical Bioscience Plasmid Mini-Prep Kit is designed for isolation of high-purity plasmid or cosmid DNA from bacterial cells for subsequent amplification, sequencing, restriction digests or transformations. The 2-step alkaline lysis procedure and binding column based preparation provide a fast, easy and efficient way of DNA isolation without shearing or significant loss of product. It allows elution in a small volume of low-salt buffer. Time-consuming phenol-chloroform extraction or alcohol precipitation are not required. The Lysis Buffer contains an integrated pH indicator to easily control the optimal pH value for DNA binding. Efficient DNA binding (for Column loading) requires a pH of 7.5 that is indicated by a color change to bright yellow. The kit can either be used in micro-centrifuges or on vacuum manifolds. It enables the extraction of plasmid DNA up to 10 kb length and yields up to 20 ?g DNA per preparation. The eluted high-quality plasmid DNA is ready to use for a variety of down-stream applications. For subsequent in vitro translation we recommend adding RNase Inhibitor or the application of an additional spin-column or phenol-chloroform based purification step. This avoids any risk of carry-over contamination with RNase due to the previous neutralization step.