RTScript™ cDNA Transcriptase
(20,000 Units)
Empirical’s RTScript™ is sensitive, specific and capable of synthesizing highly-structured and long cDNA fragments. Produced cDNA is appropriate for both PCR and qPCR analysis.
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Empirical’s RTScript™ is sensitive, specific and capable of synthesizing highly-structured and long cDNA fragments. Produced cDNA is appropriate for both PCR and qPCR analysis.
Empirical’s RTScript™ is sensitive, specific and capable of synthesizing highly-structured and long cDNA fragments. Produced cDNA is appropriate for both PCR and qPCR analysis.
The MasterMix has been developed to provide increased confidence when working with complex clinical and environmental samples.
The MasterMix has been developed to provide increased confidence when working with complex clinical and environmental samples.
The QuantiTASE PLUS Kit from Empirical Bioscience is an RT-qPCR Universal Kit contains the reagents and MasterMixes necessary for accurately and efficiently synthesizing and amplifying cDNA from RNA samples. The reagents have been optimized for qPCR or RT-qPCR applications for use with both intercalating dye and Probe-based detection methods.
The QuantiTASE PLUS Kit is a high performance version of the Empirical Bioscience QuantiTASE RT-qPCR Universal Kit. The kit contains an aptamer modified warm start version of Empirical RTScript (M-MLV) Reverse Transcriptase Enzyme mix, InhibiTaq PLUS HotStart qPCR MasterMix, inter-calculating dye and supplemental ROX reference dye.
The QuantiTASE PLUS kit from Empirical Bioscience is an RT-qPCR Universal kit contains the reagents and mastermixes necessary for accurately and efficiently synthesizing and amplifying cDNA from RNA samples. The reagents have been optimized for qPCR or RT-qPCR applications for use with both intercalating dye and Probe-based detection methods.
The QuantiTASE PLUS kit is a high performance version of the Empirical Bioscience QuantiTASE RT-qPCR Universal Kit. The kit contains an aptamer modified warm start version of Empirical RTScript (M-MLV) Reverse Transcriptase Enzyme mix, InhibiTaq PLUS HotStart qPCR Mastermix, inter-calculating dye and supplemental ROX reference dye.
The Magnesium Chloride is supplied as a 25 mM solution. This product is free of any DNase or RNase activity and is also free of any contaminating E.coli or Human genomic DNA.
Empirical’s qPCR Probe MasterMix is a 2x ready-to-use MasterMix for quantitative real time evaluation of DNA using fluorescent probe based detection. Empirical’s qPCR Probe MasterMix contains FlashTaq HotStart DNA Polymerase; a chemically modified Taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95°C. Empirical’s qPCR Probe MasterMix includes dNTPs, MgCl2, and optimized buffer for fast, efficient qPCR. Empirical’s qPCR Probe MasterMix has been optimized for use with hydrolysis based probes such as TaqMan, but is also suitable with other probe based detection systems.
Empirical’s qPCR Probe MasterMix is a 2x ready-to-use MasterMix for quantitative real time evaluation of DNA using fluorescent probe based detection. Empirical’s qPCR Probe MasterMix contains FlashTaq HotStart DNA Polymerase; a chemically modified Taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95°C. Empirical’s qPCR Probe MasterMix includes dNTPs, MgCl2, and optimized buffer for fast, efficient qPCR. Empirical’s qPCR Probe MasterMix has been optimized for use with hydrolysis based probes such as TaqMan, but is also suitable with other probe based detection systems.
Phu-50 High-Fidelity™ Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.
Phu-50 High-Fidelity™ Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Phu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings.
Phu-50 High-Fidelity™ Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’?3’ direction but does not possess a 5’?3’ exonuclease replacement activity. Its inherent 3’?5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.
Phu-50 High-Fidelity™ Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Phu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings.
Empirical’s FlashTaq HotStart DNA Polymerase is a chemically modified Taq DNA polymerase designed for reducing non- specific DNA amplification due to primer-dimer formation. FlashTaq HotStart is completely suppressed prior to activation, and regains full enzymatic activity in only 2 min at 94°C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus.
Empirical’s FlashTaq HotStart DNA Polymerase is a chemically modified Taq DNA polymerase designed for reducing non- specific DNA amplification due to primer-dimer formation. FlashTaq HotStart is completely suppressed prior to activation, and regains full enzymatic activity in only 2 min at 94°C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus.
Empirical’s FlashTaq™ HotStart 2X MasterMix is a 2X ready-to-use MasterMix that contains FlashTaq HotStart DNA Polymerase; a chemically modified HotStart Taq DNA polymerase with an activation time of 2 minutes at 94°C, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 3mM MgCl2. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C).
Empirical’s FlashTaq HotStart 2X MeanGreen™ MasterMix is a 2X ready-to-use MasterMix that contains FlashTaq HotStart DNA Polymerase; a chemically modified HotStart Taq DNA polymerase with an activation time of 2 minutes at 94°C, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 3mM MgCl2. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus.
FlashTaq HotStart 2X MeanGreen™ MasterMix contains loading dyes (blue ~4kb and yellow <25bp) that make monitoring process during electrophoresis easy, and a density agent that allows reactions to be loaded directly onto agarose gels. These dyes do not obscure visualization of reactions, as the bands run outside most products. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4C)
Samples are available. Contact us today to discuss your requirements. Call 877.479.9949 for more information.