New FlashTaq HotStart from Empirical Bioscience Offers Specificity and Stability for PCR.

Empirical Bioscience, (formerly known as Syzygy Biotech™), a company that produces high-grade PCR reagents and enzymes, announced today that it has introduced FlashTaq HotStart Hot-Start Taq DNA Polymerase, a chemically-modified Taq polymerase designed to reduce non-specific amplification in PCR. The enzyme offers, fewer contamination issues and fast activation time. In fact, under standard hot start conditions it can be activated and the enzyme recovers both 5’-3’ polymerase and 5’-exonuclease activities within two minutes versus the ten minutes of activation time required by many competitors’ enzymes.

“Unlike traditional non-hot start Taq which can become active at room temperature and begin to amplify non-specifically and form primer-dimers, FlashTaq HotStart will not become active until the temperature and time threshold has been reached, thus reducing the likelihood of non-specific amplification and primer-dimer formation,” said Pushpaja Dodla, Molecular Biologist, Empirical Bioscience.

In addition to its stability and rapid activation time, there are other factors that help FlashTaq HotStart streamline researchers’ studies, including the type of modifying reagent that it uses. The enzyme in FlashTaq HotStart has a reduced risk of contamination because it has been chemically-modified versus antibody-modified. Enzymes that are antibody-modified are highly susceptible to contamination from the organism in which they are produced.

Read more at The Scientist…