Empirical Bioscience has launched its new EB Pure Plasmid Mini-Prep Kit, which combines the firm’s High-Bind technology with alkaline-SDS lysis of bacterial cells. The kit’s High-Bind technology reversibly binds DNA or RNA under optimized conditions, removing proteins and other contaminants. It requires one to five milliliters of bacterial culture and delivers results in less than 30 minutes. It also isolates high-quality plasma or cosmid DNA for extraction up to 10 kilobases and yields up to 20 micrograms per preparation, the firm said.
The kit features an integrated pH indicator within the lysis buffer, which changes color to bright yellow when the lysis buffer reaches the pH level needed for DNA binding. The kit also includes a neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. The components create a flexible kit for downstream applications, including automated fluorescent DNA sequencing and restriction enzyme digestion, Empirical added.