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- Protease K
Protease K
Performance:
Empirical Bioscience NGS Grade protease K is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with very high specific activity for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising integrity of isolated DNA or RNA. It is a recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.
NGS Grade Protease K undergoes extra purification steps which result in increased solubility (2.5 fold), increased specific activity, and decreased DNA content, compared to standard Molecular Biology Grade products.
Proteinase K is active under wide range of reaction conditions, including elevated temperatures and presence of SDS.
Proteinase K is a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents.
Applications:
- Purification of target material from contaminated proteins
- DNA & RNA Extraction
- DNAse & Rnase removal during nucleic acid isolation
- Determination of enzyme localization- Improving cloning efficiency of PCR products
Features:
- Ultrapure with low host DNA: or ? 0.1 pg/mg NGS
- High Activity
- Active at high temperatures: <56C
- Active in the presence of SDS, Urea, etc.
- Stable over a wide Ph Range
- Supplied Lyophilized in Powder
Protein Concentration:
- 45U/mg
- One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 ?mol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.