Phi 29 Polymerase
Empirical Bioscience’s PHI 29 DNA polymerase is a recombinant protein purified from the E. coli cloned gene encoding the DNA polymerase from PHI 29 phage. Empirical’s PHI 29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage PHI 29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase.
Empirical Bioscience’s PHI 29 DNA polymerase contains a 3’- 5’ exonuclease activity that enables proofreading capability.
Phi29 DNA Polymerase exhibits highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA).
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample.
PHI 29 DNA Polymerase has very high-fidelity due to its inherent 3’-5’ exonuclease activity and can amplify from very small amounts of starting templates.
- Rolling-circle amplification (RCA)
- Multiple displacement amplification (MDA)
- Whole genome amplification (WGA)
- Preparation of DNA template for sequencing
Source of Protein:
- A recombinant E. coli strain carrying the cloned BST Prime strain
- One unit is defined as the amount of the enzyme required to catalyze the vincorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74 °C.
- 10 units/?l
- High processivity
- Proof Reading Activity: 10 to 20X Taq
- Generates Large Fragments: >10kb
- Multistrand displacement amplification
- Minimal template Required: >10ng