PCR, qPCR and RT
PFU-50 High Fidelity Polymerase
- PFU-50 High Fidelity Polymerase
(100 Units)$75.11Pfu-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-50 Polymerase-generated PCR fragments are blunt. Pfu-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings. - PFU-50 High Fidelity Polymerase
(200 Units)$146.00Pfu-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’?3’ direction but does not possess a 5’?3’ exonuclease replacement activity. Its inherent 3’?5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-50 Polymerase-generated PCR fragments are blunt. Pfu-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings. - PFU-50 High Fidelity Polymerase
(500 Units)$300.46Pfu-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex —>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-50 Polymerase-generated PCR fragments are blunt. Pfu-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings. - PFU-50 High Fidelity Polymerase
(1000 Units)$568.43Pfu-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-50 Polymerase-generated PCR fragments are blunt. Pfu-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings. - PFU-50 High Fidelity Polymerase
(5000 Units)$2,685.83Pfu-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-50 Polymerase-generated PCR fragments are blunt. Pfu-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings.
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