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Kits

KITS: Plasmid Mini-Prep

Empirical Bioscience Plasmid Mini-Prep Kit is designed for isolation of high-purity plasmid or cosmid DNA from bacterial cells for subsequent amplification, sequencing, restriction digests or transformations. The 2-step alkaline lysis procedure and binding column based preparation provide a fast, easy and efficient way of DNA isolation without shearing or significant loss of product. It allows elution in a small volume of low-salt buffer. Time-consuming phenol-chloroform extraction or alcohol precipitation are not required. The Lysis Buffer contains an integrated pH indicator to easily control the optimal pH value for DNA binding. Efficient DNA binding (for Column loading) requires a pH of 7.5 that is indicated by a color change to bright yellow. The kit can either be used in micro-centrifuges or on vacuum manifolds. It enables the extraction of plasmid DNA up to 10 kb length and yields up to 20 ?g DNA per preparation. The eluted high-quality plasmid DNA is ready to use for a variety of down-stream applications. For subsequent in vitro translation we recommend adding RNase Inhibitor or the application of an additional spin-column or phenol-chloroform based purification step. This avoids any risk of carry-over contamination with RNase due to the previous neutralization step.

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KITS: PCR Purification

Empirical Bioscience PCR Purification Kit is designed for the clean-up of PCR reactions by removal of primer dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify linear or circular DNA in the size range from 100 bp to 10 kb and is optimized for working with DNA amounts of up to 20 µg. The preparation requires no organic extractions or precipitation and guarantees high and stable recovery rates.

KITS: Agarose Gel Extraction

Empirical Bioscience Agarose Gel Extraction Kit is designed to extract high-yield DNA from agarose gels with simultaneous removal of primer dimers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify DNA in the size range from 100 bp to 10 kb. It requires no organic extractions or precipitation and guarantees high and stable recovery rates.

KITS: EB Pure Plasmid Mini-Prep Kit

EB Pure Plasmid Mini-prep Kit combine the power of High-Bind technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA in less than 30 minutes. The key to this system is the High-Bind matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. Then nucleic acids are easily eluted with deionized water or a low salt buffer. The EB Pure Plasmid Mini-prep Kit is used to isolate plasmid DNA from 1-5mL cultures. Purified plasmid DNA can be directly used for most downstream applications including automated fluorescent DNA sequencing and restriction enzyme digestion

KITS: EB Pure Agarose Gel Extraction Kit

EB Pure Agarose Gel Extraction Kit is designed for extracting desired DNA fragments from Agarose Gel. The key to this system is the high binding matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. Then nucleic acids are easily eluted with deionized water or a low salt buffer. This kit is used to recover DNA fragments between 70 bp and 20 kb with yields exceeding 85%. The recovered DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions.

KITS: EB Pure PCR Purification Kit

EB Pure PCR Purification Kit is a convenient system for the fast and reliable purification of PCR products. The key to this system is the High-Bind matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. Then nucleic acids are easily eluted with deionized water or a low salt buffer. This kit is used to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. Purified DNA can be directly used for most downstream applications uncluding T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.

KITS: EB Pure Tissue DNA Kit

EB Pure Tissue DNA Kit is a convenient system for the fast and reliable purification of genomic DNA from animal tissue. The key to this system is the High-Bind matrix that specifically, but reversibly, binds DNA under optimized conditions allowing proteins and other contaminants to be removed. The DNA is easily eluted with deionized water or a low salt buffer. There is no need for phenol/chloroform extractions, and time-consuming steps such as alcohol precipitation are eliminated. This kit is used to purify genomic DNA which is then ready for applications such as PCR, Southern blotting, and restriction enzyme digestion.

KITS: EB Pure Total RNA Kit

EB Pure Total RNA Kit is a convenient system for the fast and reliable purification of RNA from cells or soft tissue. The key to this system is the High-Bind matrix that specifically, but reversibly, binds RNA under optimized conditions allowing proteins and other contaminants to be removed. Then RNA is easily eluted with deionized water or a low salt buffer. Each column can bind up to 100?g of RNA. This kit is used to recover RNA ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.