Pfu-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-50 Polymerase-generated PCR fragments are blunt.
Pfu-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase. ERPfu-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings.