Empirical’s FlashTaq HotStart 2x MeanGreen Master Mix is a 2x ready-to-use master mix that contains FlashTaq HotStart DNA Polymerase; a chemically modified HotStart Taq DNA polymerase with an activation time of 2 minutes at 94°C, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 3mM MgCl2. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus.
FlashTaq HotStart 2X MeanGreen Master Mix contains loading dyes (blue ~4kb and yellow <25bp) that make monitoring process during electrophoresis easy, and a density agent that allows reactions to be loaded directly onto agarose gels. These dyes do not obscure visualization of reactions, as the bands run outside most products.
- 10 Reactions at 50uL per Reaction
- 25 Reactions at 20uL per Reaction
- 50 Reactions at 10uL per Reaction
STORAGE & HANDLING: Store at -20°C upon arrival. Minimize number of freeze thaw cycles by storing in working aliquots.