EB Pure Plasmid Mini-prep Kit combine the power of High-Bind technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA in less than 30 minutes. The key to this system is the High-Bind matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. Then nucleic acids are easily eluted with deionized water or a low salt buffer. The EB Pure Plasmid Mini-prep Kit is used to isolate plasmid DNA from 1-5mL cultures. Purified plasmid DNA can be directly used for most downstream applications including automated fluorescent DNA sequencing and restriction enzyme digestion.
STORAGE & HANDLING: Store at room temperature upon arrival. Solution I once the RNase A has been added should be stored 2-8ºC. Solution II must be tightly capped when not in use.