Empirical’s 5X MeanGreen Buffer is supplied as a 5X reaction buffer with 7.5mM MgCl2. 5X MeanGreen Buffer contains loading dyes (blue ~4kb and yellow <25bp) that make the monitoring process during electrophoresis easy, and a density agent that allows reactions to be loaded directly onto agarose gels. These dyes do not obscure visualization of reactions, as the bands run outside most products.
STORAGE & HANDLING: Store at -20°C upon arrival. Minimize number of freeze thaw cycles by storing in working aliquots.