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PHU-50 High Fidelity Polymerase
(1000 Units)

  • Description
  • More Info
  • PIS
  • MSDS
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Description

PHU-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered PHU DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. PHU-50 Polymerase-generated PCR fragments are blunt.

CONCENTRATION: 2.5 Units/µL

STORAGE & HANDLING: Store at -20°C upon arrival.

Additional information

Model Number

PHU-50-1000

BROCHURE: PHU-50 High Fidelity Polymerase (PDF)

PIS: PHU-50 High Fidelity Polymerase (PDF)
MSDS: PHU-50 High Fidelity Polymerase (PDF)

PHU-50 Pol: 2.5 units/µl PHU-50 Polymerase in storage buffer (50 % Glycerol, 50 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 1 mM DTT 0.1 % Tween 20, 0.1 % Nonidet P-40)
10X PHU-50 Buffer

$568.43

PHU-50 Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered PHU DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’—>3’ direction but does not possess a 5’—>3’ exonuclease replacement activity. Its inherent 3’—>5’ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. PHU-50 Polymerase-generated PCR fragments are blunt.

PHU-50 Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard PHU polymerase. ERPHU-50 Polymerase = 0.25 x 10-6 The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings.

SKU: PHU-50-1000 Categories: PHU-50 High Fidelity Polymerase, Research and Education Tags: PHU-50-1000, RESEARCH & EDUCATION

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PHU-50 High Fidelity Polymerase
(500 Units)
PHU-50 High Fidelity Polymerase
(5000 Units)
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