Syzygy Biotech™, a company that produces high-grade PCR reagents and enzymes, announced today that it has introduced rEVAlution qPCR Master Mix, an environmentally-safe, ready to use hot-start qPCR mixture specifically formulated with high quality components to offer superior safety, speed, and signaling capabilities.
EvaGreen® dye was purposely selected for this formulation because it is cell membrane impermeable making it a non-mutagenic, non-cytotoxic PCR dye that’s safe for aquatic life, safe for handling, and can be directly disposed of down the drain. Similar master mixes typically utilize SYBR® green dyes that are mutagenic. EvaGreen® also helps rEVAlution produce signals that are stronger than the SYBR® green dyes. As a Syzygy Biotech customer noted “I compared rEVAlution [to] a competitor SYBR®, and found CT values that are very similar, but [the] signals are four times stronger.”
In addition, Syzygy FlashTaq HotStart, a chemically-modified hot-start Taq DNA polymerase recognized for its rapid activation and high level of compatibility was selected for use in the formulation.