Empirical’s QuantiTASE Two-Step Reagent Kit contains all the components necessary to perform first strand cDNA synthesis with high sensitivity and efficiency using a two step RT-qPCR protocol. These reagents combined with the optimized buffers and reagents in the QuantiTASE kits synthesizes complementary DNA from single stranded RNA or DNA templates. Empirical’s RTScript™ is sensitive, specific, and is capable of synthesizing highly structured and long cDNA fragments. Produced cDNA is appropriate for qPCR analysis.
Empirical QuantiTASE Two-Step Reagent Kit is supplied with 5X RTScript™ Buffer, dNTP Mix (10mM) , DTT Stock Solution (100mM), Oligo-(dT)20 primer (100?M), Random Hexamers (100?M), RNase Inhibiter (40 units/?L), Positive Control RNA (10ng/?L), and RNase-free Water.