Taq DNA Polymerase
The Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E.coli. The recombinant Taq DNA Polymerase shows identical characteristics to native Taq from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa, and will synthesize DNA products having dA overhangs on the 3’ ends. This product has been optimized to allow prolonged use and storage at Ambient Temperature (18C+/-4C) and in standard Refrigeration (4C)
Empirical Taq DNA Polymerase is supplied in a storage buffer containing: 20mM HEPES (pH 7.9), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% (v/v) Glycerol, and other stabilizers.
CONCENTRATION: 5U/µl
STORAGE & HANDLING:
Long Term Storage at -20C Recommended
Up to 30 Days at 18C
Up to 90 Days at 4C
Taq 2X Master Mix
Empirical’s Taq 2X Master Mix is supplied in 2X reaction buffer with 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, 3mM MgCl2 and Taq DNA polymerase. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)
- 500 Reactions at 50uL per Reaction
- 1250 Reactions at 20uL per Reaction
- 2500 Reactions at 10uL per Reaction
CONCENTRATION: 2X
STORAGE & HANDLING:
Long Term Storage at -20C Recommended.
Minimize number of Freeze Thaw cycles of Frozen Product
Up to 60 Days at 15° to 25°C
Up to 180 Days at 4°C
FlashTaq HotStart 2X Master Mix
Empirical’s FlashTaq HotStart 2x Master Mix is a 2x ready-to-use master mix that contains FlashTaq HotStart DNA Polymerase; a chemically modified HotStart Taq DNA polymerase with an activation time of 2 minutes at 94°C, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 3mM MgCl2. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)
- 100 Reactions at 50uL per Reaction
- 250 Reactions at 20uL per Reaction
- 500 Reactions at 10uL per Reaction
CONCENTRATION: 2X
STORAGE & HANDLING:
Long Term Storage at -20C Recommended. Minimize number of Freeze Thaw cycles of Frozen Product
Up to 60 Days at 15° to 25°C
Up to 180 Days at 4°C
5X Multiplex Master Mix
Empirical’s 5x Multiplex Master Mix is an easy-to-use master mix that contains optimized high quality enzyme and salt concentrations combined with optimized stabilizers which allow for maximum detection of two or more products in one PCR reaction simultaneously.
This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)
- 100 Reactions at 50uL per Reaction
- 250 Reactions at 20uL per Reaction
- 500 Reactions at 10uL per Reaction
CONCENTRATION: 5X
STORAGE & HANDLING:
Long Term Storage at -20C Recommended. Minimize number of Freeze Thaw cycles of Frozen Product
Up to 60 Days at 15° to 25°C
Up to 180 Days at 4°C
Taq 2X MeanGreen Master Mix
Empirical’s Taq 2X MeanGreen Master Mix is supplied in 2X reaction buffer with 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, 3mM MgCl2 and Taq DNA polymerase. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)
- 100 Reactions at 50uL per Reaction
- 250 Reactions at 20uL per Reaction
- 500 Reactions at 10uL per Reaction
CONCENTRATION: 2X
STORAGE & HANDLING:
Long Term Storage at -20C Recommended. Minimize number of Freeze Thaw cycles of Frozen Product
Up to 60 Days at 15° to 25°C
Up to 180 Days at 4°C
FlashTaq HotStart 2X MeanGreen Master Mix
Empirical’s FlashTaq HotStart 2x MeanGreen Master Mix is a 2x ready-to-use master mix that contains FlashTaq HotStart DNA Polymerase; a chemically modified HotStart Taq DNA polymerase with an activation time of 2 minutes at 94°C, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 3mM MgCl2. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. The recombinant Taq DNA polymerase shows identical characteristics to native Taq from Thermus aquaticus. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4°C)
- 100 Reactions at 50uL per Reaction
- 250 Reactions at 20uL per Reaction
- 500 Reactions at 10uL per Reaction
CONCENTRATION: 2X
STORAGE & HANDLING:
Long Term Storage at -20C Recommended. Minimize number of Freeze Thaw cycles of Frozen Product
Up to 60 Days at 15° to 25°C
Up to 180 Days at 4°C
rEVAlution 2X qPCR Master Mix
Empirical’s rEVAlution is a ready to use hot-start qPCR Master Mix with EvaGreen® dye, FlashTaq HotStart DNA polymerase, 400µM dCTP, 400µM dGTP, 400µM dATP, 400µM dTTP, and 5mM MgCl2. EvaGreen® is a dsDNA-binding dye. It is cell membrane impermeable, non-cytotoxic, non-mutagenic, and safe for aquatic life. EvaGreen® possesses similar absorption and emission spectra as SYBR® Green I. This product has been optimized to allow prolonged use and storage at Ambient Temperature (15° to 25°C) and in standard Refrigeration (4C)
- 100 Reactions at 20uL per Reaction
- 200 Reactions at 10uL per Reaction
CONCENTRATION: 2X
STORAGE & HANDLING:
Long Term Storage at -20C Recommended. Minimize number of Freeze Thaw cycles of Frozen Product
Up to 60 Days at 15° to 25°C
Up to 180 Days at 4°C