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Kits

KITS: Plasmid Mini-Prep

Empirical Bioscience Plasmid Mini-Prep Kit is designed for isolation of high-purity plasmid or cosmid DNA from bacterial cells for subsequent amplification, sequencing, restriction digests or transformations. The 2-step alkaline lysis procedure and binding column based preparation provide a fast, easy and efficient way of DNA isolation without shearing or significant loss of product. It allows elution in a small volume of low-salt buffer. Time-consuming phenol-chloroform extraction or alcohol precipitation are not required. The Lysis Buffer contains an integrated pH indicator to easily control the optimal pH value for DNA binding. Efficient DNA binding (for Column loading) requires a pH of 7.5 that is indicated by a color change to bright yellow. The kit can either be used in micro-centrifuges or on vacuum manifolds. It enables the extraction of plasmid DNA up to 10 kb length and yields up to 20 ?g DNA per preparation. The eluted high-quality plasmid DNA is ready to use for a variety of down-stream applications. For subsequent in vitro translation we recommend adding RNase Inhibitor or the application of an additional spin-column or phenol-chloroform based purification step. This avoids any risk of carry-over contamination with RNase due to the previous neutralization step.

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KITS: PCR Purification

Empirical Bioscience PCR Purification Kit is designed for the clean-up of PCR reactions by removal of primer dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify linear or circular DNA in the size range from 100 bp to 10 kb and is optimized for working with DNA amounts of up to 20 µg. The preparation requires no organic extractions or precipitation and guarantees high and stable recovery rates.

KITS: Agarose Gel Extraction

Empirical Bioscience Agarose Gel Extraction Kit is designed to extract high-yield DNA from agarose gels with simultaneous removal of primer dimers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify DNA in the size range from 100 bp to 10 kb. It requires no organic extractions or precipitation and guarantees high and stable recovery rates.