PRESS RELEASE: New High Fidelity Blunt-End PFU-50 Polymerase from Empirical Bioscience Offers Precise, Rapid Amplification

Empirical Bioscience announced today that they have introduced PFU-50, an ultra high fidelity Polymerase for PCR application. In addition to delivering a fidelity that is 50 times greater than Taq polymerases and a rate of accuracy that is 2-fold higher than standard Pfu polymerases, PFU-50 also offers improved processing times, shorter elongation times, and better ligation.
The genetically engineered enzyme invokes the polymerization of nucleotides into duplex DNA in 5’?3’ direction but does not possess 5’?3’ exonuclease replacement activity. Instead, it offers a 3’?5’ exonuclease proofreading capability, significantly increasing the fidelity of DNA compared to Taq polymerase. This extensive exonuclease activity leaves the PCR fragments generated by PFU-50 High Fidelity Polymerase blunt, and without overhangs, for more effective linking of DNA. These characteristics lend themselves well to applications like cloning and next generation sequencing where accuracy, simple ligation, and speed are key.

“PFU-50 is ideal for applications that demand accurate replication of DNA,” said Marian Willing, Lead Scientist at Empirical Bioscience. “As well as those featuring long or difficult amplifications,” she added.

PFU-50 can be purchased on Empirical Bioscience’s Web site in units of 100 to 5,000. Free samples are also available. Empirical also offers this product, and all other products in bulk quantities

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LABORATORY EQUIPMENT: Gel Extraction Kit Streamlines DNA Purification

Empirical Bioscience’s Agarose Gel Extraction Kit offers a simplified DNA purification process and robust results. Specifically designed to enhance efficiency, the kit arrives with the necessary components including extraction, activation, washing and elution buffers, as well as spin columns and collection tubes. The kit allows for efficient DNA purification from agarose gels and provides maximum recovery of DNA from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide and other impurities.

Continue reading at Laboratory Equipment.

GENOME WEB: Empirical Bioscience Plasmid Mini-Prep Kit

Empirical Bioscience recently introduced its Plasmid Mini-Prep Kit. The kit requires only 1 to 3 mL of bacterial culture, and isolates for extraction high-quality plasma or cosmid DNA up to 10 kb in length with yields of up to 20 µg per preparation. The kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches 7.5, the optimal pH level for DNA binding. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.

Continue reading at Genome Web.

BIOSPACE: Empirical Bioscience’s Plasmid Mini-Prep Kit Delivers Maximum Yields Using Minimal Culture

Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation.

In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.

Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in micro-centrifuges or on vacuum manifolds.

Continue reading at BioSpace.

GENETIC ENGINEERING & BIOTECHNOLOGY: New Products – Agarose Gel Extraction Kit

The new Agarose Gel Extraction Kit offers a simplified DNA purification process designed to enhance efficiency. The kit is supplied with the necessary components to streamline extraction workflows including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. The new kit allows for efficient DNA purification from agarose gels. The kit provides maximum recovery of DNA from 100 bp to 10 kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer so no organic extractions or additional precipitations are necessary—helping to insure high and consistent recovery rates.

Continue reading at GEN

BIO PORTFOLIO: Empirical Bioscience’s New Agarose Gel Extraction Kit Streamlines and Maximizes DNA Purification

Empirical Bioscience announced today that they have introduced a new Agarose Gel Extraction Kit that offers a simplified DNA purification process and robust results.

Specifically designed to enhance efficiency, the kit arrives with the necessary components including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes.

Continue reading at Bio Portfolio.

LAB BULLETIN: Empirical Bioscience’s New Agarose Gel Extraction Kit Streamlines and Maximizes DNA Purification

Empirical Bioscience announced today that they have introduced a new Agarose Gel Extraction Kit that offers a simplified DNA purification process and robust results.

Specifically designed to enhance efficiency, the kit arrives with the necessary components including, extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. Empirical Bioscience’s Agarose Gel Extraction Kit allows for efficient DNA purification from agarose gels. The kit provides maximum recovery of DNA from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities.

Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer so no organic extractions or additional precipitations are necessary. This helps to guarantee high and consistent recovery rates.

Continue reading at Lab Bulletin.

PRESS RELEASE: Empirical Bioscience Plasmid Mini-Prep Kit Delivers Maximum Yields Using Minimal Culture

Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation.

In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.

Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in micro-centrifuges or on vacuum manifolds.

“The ability to use less bacterial culture to purify a sequence away from the genome and to refine the mixture for optimal DNA or plasmid isolation are significant advantages,” said Des O’Farrell, President of Empirical Bioscience. “Our kit has been shown to produce more high-quality plasmid than competitor brands that use more culture,” he added.

Empirical Bioscience produces high-grade PCR reagents and enzymes in its ISO 13485 certified facility in Grand Rapids, MI, USA and is a Registered Small Business Company.
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PRESS RELEASE: PCR Kit from Empirical Bioscience Simplifies and Optimizes DNA Purification

Empirical Bioscience announced today that they are now offering a PCR Purification Kit that streamlines the DNA purification process and delivers high-yield recovery.

Designed with efficiency in mind, the kit arrives with the essential components including, binding buffer, activation buffer, elution buffer, washing buffer, spin columns, and collection tubes. Empirical Bioscience’ PCR Purification Kit allows for efficient DNA purification. The kit provides maximum recovery of linear or circular DNA ranging in size from 100bp to 10kb while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, loading dyes, and other impurities.

Based on silica-membrane technology, the extraction kits allow for the binding of DNA in high-salt and elution in a low-salt buffer, and is designed to work with DNA amounts of up to 20 µg, so no organic extractions or additional precipitations are necessary. This helps to guarantee high and stable recovery rates.

“Our customer’s needs are our highest priority, and typically two of their main concerns are yield and efficiency,” said Des O’Farrell, President of Empirical Bioscience. “That’s why we kept ease-of-use and optimal recovery top-of-mind when we created this kit,”

Empirical Bioscience produces high-grade PCR reagents and enzymes in its ISO 13485 certified facility in Grand Rapids, MI, USA and is a Registered Small Business Company.

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GENOME WEB: Empirical Bioscience Agarose Gel Extraction Kit

Empirical Bioscience has launched the Agarose Gel Extraction kit. The kit includes an extraction buffer, activation buffer, washing buffer, elution buffer, spin columns, and collection tubes. It allows efficient DNA purification from agarose gels and provides maximum recovery of DNA from 100bp to 10kb. It also removes primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities, the company said.

Continue reading at Genome Web.